|
Mitochondrial genome as a powerful tool for identity
|
| |
|
|
|
Abstract: (18166 Views) |
| Abstract
Background & Aims: Human mitochondrial DNA (mtDNA) is a double-stranded circular molecule present in 1,000 to 10,000 copies per cell. The complete nucleotide sequence of the 16,569 base-pair molecule was determined in 1981.The aim of this study is to assess polymorphism, the rate of mutation, homoplasy, haplogroups frequency , haplotypes variance and nucleotide diversity in HVS-1 region of mtDNA from a portion of the Iranian ethnic groups.
Materials & Methods: In present study, blood samples were randomly obtained from 357 healthy unrelated volunteer according to their place of birth and their information’s records for Persian, Azerbaijanian, Gilaki, Kurdish, Baluchi, Sistain, Turkmen and Arabs. Genomic DNA was extracted from blood following salting out method. Amplification of HVS-1 region was carried out using one set of primers encompassing the HVS-1 region. Automated DNA sequencing was carried out using dye-terminator chemistries, on an automated DNA sequencer (ABI 310). The sequences were edited for HVS-1 and aligned with the Cambridge reference sequence by clustalx program. According to mtDNA phylogenetic tree the haplogroups were determined.
Findings: A total of 160 polymorphic sites were observed, in 357 HVS-1 templates. The highest homoplasy (similar mutation in individuals) with 40% belongs to Persians and the lowest with 13% was seen in Sistains. The rate of nucleotide diversity in Persian is 0.862 and it is the lowest diversity in all studied ethnic groups. We found that the variance of haplotype in Sistain was the highest with 0.87.The most common haplogroup in Persian, Azerbaijanian, Gilaki, Kurdish, Sistain, was HV and in Baluchi,Turkmen and Arabs were M and N.
Conclusion: According to our results, contrary to our initial assumption, since Iran is situated in Asian continent, in Persian, Azerbaijanian, Gilaki, Kurdish, Sistain the haplogroups of western Eurasia were dominant and in Arabs and Baluchi, western Eurasia Haplogroups shows the equal frequency. On the other hand in Turkmen the highest frequency haplogroup belongs to eastern Eurasia. According to variance of mtDNA haplotypes in Iranian ethnic groups specially sistains, application of mtDNA in forensic investigation and identity is an effective tool. |
|
| Keywords: : Mitochondrial DNA, Haplotype, Polymorphism, Haplogroup, Identity |
|
|
Full-Text [PDF 812 kb]
(3635 Downloads)
|
Type of Article: Research Article |
Subject:
Forensic Medicine
Received: 2009/03/18 | Revised: 2009/05/6 | Accepted: 2018/03/29 | ePublished: 2018/03/29
|
|
|
|
|
|
