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:: Volume 14, Issue 3 (2008) ::
Iran J Forensic Med 2008, 14(3): 159-165 Back to browse issues page
Comparison the effectiveness of extraction protocols Phenol-Chloroform-Silica and Guanidinium Thiocyanate-Silica in recovering DNA from human skeletal remains
Abstract:   (20289 Views)
Background & Aim: DNA Typing methods offer great help in identification of single deceased individuals, war and mass disaster victims. It has been a challenge to extract DNA from bones previously soaked in water, burned or buried for a long time, due to the reduced quality and quantity of DNA in the bone samples. The aim of this investigation is the comparison and evaluation of two different DNA extraction methods, namely the phenol-chloroform-silica and the guanidinium thiocyanate-Silica for DNA analysis of old bones. Materials & Methods: DNA was extracted from twenty randomly chosen bone samples, with 8 years passing of their death, from unknown human remains by using of phenol-chloroform-silica and guanidinium thiocyanate-Silica methods. Spectrophotometer was used to estimate the quantity and quality of DNA. Then, extracted DNA was profiled by a set of eight short tandem repeats (STRs) loci named CD4, vWA, LPL, D5S818, D16S539, D13S317, F13 and FES. The successfulness of the DNA profiling of the specimens was measured by counting all type able electrophoretic results in each case. Findings: DNA quantification results showed that the phenol-chloroform-silica extracted on average 256.27 µg/ml of DNA with the average purity degree of 1.07, compared with the DNA concentration of 170.86 µg/ml and purity of 0.94 by the guanidinium thiocyanate-Silica. Our results on the overall success rate of polymerase chain reaction (PCR) amplification in eight STRs loci mentioned above indicates that phenol-chloroform-silica method (76%) was more effective than guanidinium thiocyanate-Silica (64%). Conclusions: Although the phenol-chloroform-silica showed better results in STR Typing from degraded bone samples than guanidinium thiocyanate-Silica, we suggest using both methods in analysis of old bone tissue simultaneously to improve the interpretation of results.
Keywords: DNA Profiling, Bone, Polymerase Chain Reaction, Short Tandem Repeats
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Type of Article: Research Article | Subject: Forensic Medicine
Received: 2009/03/18 | Revised: 2009/05/6 | Accepted: 2018/03/29 | ePublished: 2018/03/29


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Comparison the effectiveness of extraction protocols Phenol-Chloroform-Silica and Guanidinium Thiocyanate-Silica in recovering DNA from human skeletal remains. Iran J Forensic Med 2008; 14 (3) :159-165
URL: http://sjfm.ir/article-1-101-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 3 (2008) Back to browse issues page
مجله پزشکی قانونی ایران Iranian Journal of Forensic Medicine
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