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:: Volume 25, Issue 3 (2019) ::
Iran J Forensic Med 2019, 25(3): 121-129 Back to browse issues page
Optimization of a Dispersive Liquid-Liquid Microextration Method for Analysis of Chlorpyrifos in Urine Using the Chemometrics Method
R. Mohammadzaheri1, M. Ansari Dogaheh2, M. Kazemipour1, K. Soltaninejad 3
1- Chemistry Department, Science Faculty, Kerman Branch, Islamic Azad University, Kerman, Iran
2- Pharmaceutics Department, Pharmacy Faculty, Kerman University of Medical Sciences, Kerman, Iran
3- Forensic Toxicology Department, Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran , kamsoltaninejad@gmail.com
Abstract:   (700 Views)
Aims: The use of simple, low cost, high-efficiency microextraction methods are considered for sample preparation in forensic toxicology. Nowadays, the chemometrics technique can be used to determine the important and influencing factors on the response, to optimize the extraction methods. The aim of this study was to design and optimize a dispersive liquid-liquid microextraction (DLLME) method for the extraction of chlorpyrifos from the urine using a chemometrics method.
Materials & Methods: In this experimental study,  at first, a DLLME method for the extraction of chlorpyrifos in the urine sample was designed. Then, the Taguchi model was used for screening and investigating the role of effective factors on the extraction of chlorpyrifos from the urine and a central composite design was used to examine the interaction of these factors. After validation of the extracted data, chlorpyrifos was extracted from the urine using an optimized DLLME method and it detected and quantified using the high-performance liquid chromatography with photodiode array detector (HPLC-PDA).
Findings: The optimized DLLME-HPLC-PDA method was linear in the range of 0.5 to 4μg/ml, and the R2 coefficient was 0.9996. The minimum rates of detection and quantification ​​were calculated by 0.08 and 0.25μg/ml, respectively. The profitability of the method in the optimal condition was calculated by %95.6.
Conclusion: The optimized DLLME-HPLC-PDA method can be used as a simple, fast, inexpensive, sensitive and precise method for chlorpyrifos analyzing in urine specimens in clinical and forensic toxicology laboratories.
Keywords: Chlorpyrifos|Chemometrics|Dispersive Liquid -Liquid Microextraction (DLLME)|High Performance Liquid Chromatography (HPLC) ,
Full-Text [PDF 809 kb]   (154 Downloads)    
Article Type: Original Research | Subject: Forensic Toxicology
Received: 2019/06/10 | Accepted: 2019/08/28 | Published: 2019/09/21
English Expanded Abstract [HTML 22 KB]  (17 Download)
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Mohammadzaheri R, Ansari Dogaheh M, Kazemipour M, Soltaninejad K. Optimization of a Dispersive Liquid-Liquid Microextration Method for Analysis of Chlorpyrifos in Urine Using the Chemometrics Method. Iran J Forensic Med. 2019; 25 (3) :121-129
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Volume 25, Issue 3 (2019) Back to browse issues page
مجله پزشکی قانونی Scientific Journal of Forensic Medicine
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