Background  : In the past decades, lip-print studies (Cheiloscopy) attracted  many scientists as a new tool for human identification in  civil, criminal issues and also genetic diseases. The lip prints are the normal lines in lip that is unique as a individually fingerprint and have patterns  which are dependent on ethnicity and gender.

Methods: In this research , after obtaining consent from cases, 96  femail person were  studied of lip lines. For performing this research, for full- face photograph of lips, a digital camera (Canon Power shot A2200, 14.1 Mega Pixel) was used. Pictures transferred to the computer and by picture manager software, the pattern of each  lip lines was studied . Then each image was divided into six areas. The frequency of each pattern (complete vertical, incomplete vertical, intersected, branch , reticular, irregular) were reported.

Findings: The most frequent pattern in the right upper lip was intersected (36/4%), in  middle upper lip, reticular pattern 35/4%, in  left upper lip branch pattern 43/7%, in  right lower lip intersected pattern 57/2%, In the middle of the lower lip complete vertical pattern 40/6%, in  left lower lip intersected pattern 62/5% were observed.

Conclusion: this study shows that in the included sampels the branched lip pattern is dominate type and incompelete vertical is rare type. Maybe a multi-gene system, as the controls lines of the skin , controls the skin lines histogenesis on the lip.