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Optimizing Bone DNA Extraction for Obtaining High Yield Genome of Ancient Teeth and Bones
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Hossein Samadi Kafil   1, Mohammad Asghar zadeh2 , Elham Zeinal zadeh3 , Seyyed Baha Hossini Sarteshnizi4 |
1- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , kafilhs@tbzmed.ac.ir
2- , Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3- Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Department of Archeology, Faculty of Humanities, Tarbiat Modarres University, Tehran, Iran. |
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Abstract: (9188 Views) |
Background: Polymerase chain
reaction (PCR) is one of important molecular methods that let us to understand more
about molecular aspects of cells. DNA is the most important part of PCR
reaction, that its quality and amount has direct effect on final results. In
this study, we aimed to develop a protocol for extracting DNA from samples of
tooth and bone belong to 3600 years ago. Method:
After grilling and cleaning all surface parts of bones and teeth we used
buffers for removing calcium and other PCR inhibitors then, we used Silica
beads for collecting all DNA in our samples. Findings: DNA extraction
amounts were 37ng/µl, 25ng/µl, 7ng/µl, 32ng/µl, 18ng/µl, 42ng/µl.
In 67% of samples, we had extractions with high purity according to nano-drop
results. Extracted DNA were examined for Real Time PCR, beta-actin gene that we
had good results. Conclusion:
Results of this study showed that this developed protocol has a good yield of
DNA with enough purity and removing PCR inhibitors. We recommend this protocol
for applying in same samples and studies. |
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| Keywords: DNA Extraction, Polymerase Chain Reaction, Ancient, Bone, Teeth, Silica |
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Full-Text [PDF 1467 kb]
(6534 Downloads)
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Type of Article: Research Article |
Subject:
Forensic Medicine
Received: 2014/07/9 | Revised: 2014/07/9 | Accepted: 2014/07/9 | ePublished: 2014/07/9
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